联系方式

办公电话:

电子邮箱:lihlin6@mail.sysu.edu.cn

办公地址:广州市广州大学城外环东路132号中山大学药学院421室

简要介绍

李惠琳,博士,中山大学教授,博导。201712月通过中山大学百人计划进入中山大学药学院工作。其研究工作以高分辨质谱技术为主要手段,开发先进的Top-Down MS技术,在整体蛋白、蛋白质机器水平上实现对蛋白的测序、蛋白翻译后修饰位点及药物结合位点的精准定位,进而对蛋白质机器结构和功能及蛋白-药物作用机制进行深入研究。其研究成果发表在Nature Chemistry, Analytical Chemistry, J. Am. Soc. Mass Spectrom.等国际主流化学、分析、及质谱类杂志。

研究方向

1)开发及利用整合结构质谱技术(包括native top-down MS, HDX-MS, cross-linking MS等)研究蛋白质机器(蛋白-药物、蛋白-蛋白、蛋白-DNA等)的结构和功能;

2)开发靶向middle-down/top-down蛋白质组学技术探索蛋白翻译后修饰(PTMs)在生命过程中的调控机制。

教育经历

200910月至201212月:博士,英国华威大学化学系,导师:Peter B. O’Connor 教授;

20059月至200812月:硕士,中国科学院长春应用化学研究所,导师:刘志强 研究员;

19989月至20027月:学士,山东大学药学院

工作经历

201712-至今 教授,博士生导师,中山大学,药学院

20169月至20179月:博士后,法国遗传与分子细胞生物学研究所结构生物学系,合作导师:Bruno Kieffer & Marc-Andre Delsuc教授;

20131月至20161月:博士后,美国加州大学洛杉矶分校David Geffen医学院生物化学系,合作导师:Joseph Loo教授;

20027月至20099月:研习员,助理研究员,中国科学院长春应用化学研究所

科研项目

中山大学百人计划二期启动项目,负责

国家自然科学基金面上项目,2019.1-2022.12,负责

论著专利

  1. H Li*. Native top-down proteomics comes into light. Sci. China Life Sci. 2018, 61(11), 1425–1427.
  2. H Li*, HH Nguyen, RRO Loo, I Campuzano, JA Loo*. An integrated native mass spectrometry and top-down proteomics method that connects sequence to structure and function of macromolecular complexes. Nat. Chem. 2018, 10(2):139-148
  3. H Li*, Y Sheng, W McGee, M Cammarata, D Holden, JA Loo*. Structural Characterization of Native Proteins and Protein Complexes by Electron Ionization Dissociation. Anal. Chem. 2017. 89, 2731-2738
  4. I Campuzano, H Li, G Svitel, D Bagal, L Jia, R Kurzeja, H Xu, JA Loo*. Bacteriorhodopsin and Nanodisc Analysis by Orthogonal Acceleration Time-of-flight, Ion Cyclotron Resonance and OrbiTrap Analyzers. Anal. Chem. 2016. 88, 12427-12436.
  5. P Wongkongkathep, H Li, X Zhang, RRO Loo, R Julian and JA Loo*. Enhancing Disulfide Bond Cleavage by UV Excitation and Electron Capture Dissociation for Top-Down Mass Spectrometry. Int. J. Mass Spectrom. 2015. 390, 137-145.
  6. X Zhang, H Li, JA Loo, RR Julian*, B Moore, P Wongkongkathep, RRO Loo. Radical directed dissociation of peptides and proteins by IRMPD and SORI-CID with FTICR mass spectrometry. Rapid Commun. Mass Spectrom. 2014. 28, 2729-2734.
  7. H Li, P Wongkongkathep, SL Van Orden, RRO Loo, JA Loo*. Revealing Ligand Binding Sites and Quantifying Subunit Variants of Non-Covalent Bound Protein Complexes in a Single Native Top-Down FTICR MS Experiment. J. Am. Soc. Mass Spectrom. 2014. 25, 2060-2068.
  8. H Li, JJ Wolff, SL Van Orden, JA Loo*. Native Top-Down ESI-MS of 158 kDa Protein Complex by High Resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Anal. Chem. 2014, 86, 317-320 (Paper On the Cover)
  9. H Li, JR Snelling, MP Barrow, JH Scrivens, PJ Sadler, PB O’Connor*. Mass Spectrometric Strategies to Improve the Identification of Pt(II)-modification Sites on Peptides and proteins. J. Am. Soc. Mass Spectrom. 2014. 25, 1217-1227.
  10. Y Zhao, NJ Farrer, H Li, JS Butler, RJ McQuitty, A Habtemariam, F Wang, PJ Sadler*. De novo generation of singlet oxygen and ammine ligands by photoactivation of a platinum anticancer complex. Angew. Chem. 2013. 52, 1-6.
  11. Y Qi, H Li, RH Wills, P Perez-Hurtado, X Yu, DPA Kilgour, MP Barrow, C LinPB O’Connor*. Absorption-mode Fourier transform mass spectrometry: The effects of apodization and phasing on modified protein spectra. J. Am. Soc. Mass Spectrom. 2013, 24, 828-834.
  12. J Wei, H Li, MP Barrow, PB O’Connor*. Structural Characterization of Chlorophyll a by High Resolution Tandem Mass Spectrometry. J. Am. Soc. Mass Spectrom. 2013. 24, 753-760.
  13. Y Qi, Z Liu, H Li, PJ Sadler, PB O'Connor*. Mapping the Protein-Binding Sites for Novel Iridium(III) Anticancer Complexes Using Electron Capture Dissociation. Rapid Commun. Mass Spectrom. 2013, 27, 2028-2032.
  14. Y Zhao, JA Woods, NJ Farrer, KS Robinson, J Pracharova, J Kasparkova, O Novakova, H Li, L Salassa, AM Pizarro, GJ Clarkson, L Song, V Brabec, PJ Sadler*. Diazido mixed-amine platinum(IV) anticancer complexes activatable by visible-light form novel DNA adducts. Chem. Eur. J. 2013, 19, 9578-9591.
  15. H Li, PB O’Connor*.Electron Capture Dissociation of Disulfide, Sulfur-Selenium, and Diselenide Bound Peptides. J. Am. Soc. Mass Spectrom. 2012, 23, 2001-2010.
  16. H Li#, SA Wells#, JE Jimenez-Roldan, RA Römer, Y Zhao, PJ Sadler, PB O’Connor*. Protein Flexibility is Key to Cisplatin Cross-linking in Calmodulin. Prot. Sci. 2012. 21, 1269-1279.
  17. Y Qi, MP Barrow, H Li, JE Meier, SL Van Orden, CJ Thompson, PB O'Connor*. Absorption-mode: The Next Generation of Fourier Transform Mass Spectra. Anal. Chem. 2012. 84, 2923–2929.
  18. H Zhou, H Li, Z Zheng, F Song, J Xing, Z Liu*, S Liu*. Screening for α-Glucosidase Inhibitors from Coptidis-rehmanniae Herb Couple by Using Ultrafiltration Liquid Chromatography/Mass Spectrometry. J. Liq. Chrom. Rel. Technol. 2012, 35, 1-14.
  19. H Li, TY Lin, SL Van Orden, Y Zhao, MP Barrow, AM Pizarro, Y Qi, PJ Sadler, PB O'Connor*. Use of Top-down and Bottom-up Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Mapping Calmodulin Sites Modified by Platinum Anticancer Drugs. Anal. Chem. 2011, 83, 9507-9515.
  20. H Li, Y Zhao, HIA Phillips, Y Qi, TY Lin, PJ Sadler, PB O’Connor*. Mass Spectrometry Evidence for Cisplatin as a Protein Cross-linking Reagent. Anal. Chem. 2011, 83, 5369-5376.
  21. Y Qi, MP Barrow, SL Van Orden, CJ Thompson, H Li, P Perez-Hurtado, PB O'Connor*. Variation of the Fourier Transform Mass Spectra Phase Function with Experimental Parameters. Anal. Chem. 2011, 83, 8477-8483.
  22. W Liu, S Liu, H Li, F Song, Z Liu, S Liu*. Binding of Alpha 1-acid Glycoprotein with Aconitum Alkaloids: An Investigation by IF-MALDI-FT-MS. Rapid Commun. Mass Spectrom. 2011, 25, 973-978.
  23. S Liu, J Yan, H Li, F Song, Z Liu, Z Liu, S Liu. Studies on Chemical Constituents of Compound Indigowoad Root Granule by Mass Spectrometry. Chem. J. Chinese U. 2010, 31, 1137-1142.
  24. H Li, F Song, J Xing, Z Liu*, S Liu*. Screening and Structures Characterization of α-Glucosidase Inhibitors from Hawthorn Leaves Flavonoids Extract by Ultrafiltration LC-DAD-MSn and SORI-CID FT ICR MS. J. Am. Soc. Mass Spectrom. 2009, 20, 1496-1503.
  25. X Meng, H Li, F Song, C Liu, Z Liu, S Liu. Studies on Triterpenoids and Flavones in Glycyrrhiza uralensis Fisch. by HPLC-ESI-MSn and FT-ICR-MSn. Chinese J. Chem. 2009, 27, 299-305.
  26. H Li, F Song, Z Zheng, Z Liu*, S Liu*. Characterization of Oligsaccharides and Phenolid Acids in the Chinese Herb Tanshen by ESI-FT ICR MS. J. Mass Spectrom. 2008, 43, 1545-1552.
  27. H Yue, Z Pi, H Li, F Song, Z Liu, S Liu. Studies on the Stability of Diester-diterpenoid Alkaloids from the Genus Aconitum L. by High Performance Liquid Chromatography Combined with Electrospray Ionisation Tandem Mass Spectrometry (HPLC/ESI/MSn). Phytochem. Anal. 2008, 19, 141-147.
  28. Y Liu, Z Liu, H Li, F Song, S Liu. Studies on Diterpenoids Constituents from Euphorbia Kansui by Electrospray Ionization Multi-Stage Tandem Mass Spectrometry. Chem. J. Chinese U. 2008, 29, 1727-1735.
  29. X Qiu, M Cui, H Li, Z Liu, S Liu. Prompt Disulfide Fragmentations of Disulfide-containing Proteins in a Matrix-Assisted Laser Desorption/ Ionization Source. Rapid Commun. Mass Spectrom. 2007, 21, 3520-3525.
  30. Y Zhao, F Song, H Yue, X Guo, H Li, Z Liu, S Liu. Deoxyaconitine of Metabolite of Aconitine by Human Intestinal Bacteria and Electrospray Ionization Tandem Mass Spectrometry. Chem. J. Chinese U. 2007, 28, 2051-2055.
  31. H Dong, Z Liu, F Song, Z Yu, H Li, S Liu. Structural Analysis of Monoterpene Glycosides Extracted from Paeonia lactiflora Pall. Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and High-performance Liquid Chromatography/ Electrospray Ionization Tandem Mass Spectrometry. Rapid Commun. Mass Spectrom. 2007, 21, 3193-3199.
  32. S Wang, H Li, Z Liu, S Liu, B Cui. Studies on the Contents of Catechin and Epicatechin in Catechu by HPLC, Lishizhen Medicine and Materia Medica Research. 2006, 17, 490-491.
  33. H Dong, Z Liu, F Song, Z Yu, H Li, S Liu. Studies on Paeoniflorin by Electrospray Ionization Tandem Mass Spectrometry. Chem. J. Chinese U. 2006, 27, 2066-2069.

获奖情况

  • AOMSC Young Scientist Travel Award (2017)
  • ASMS Postdoctoral Career Development Award (2014)
  • 2009-2012 Warwick Postgraduate Research Scholarship (WPRS) (2009-2012)
  • University of Warwick Departmental Studentships (2009-2012)
  • Travel Award for the 25th Lake Louise Tandem Mass Spectrometry Workshop (2012)
  • IMSF Travel Grant for the 19th International Mass Spectrometry Conference (2012)
  • BMSS Travel Award for the 59th ASMS Conference on Mass Spectrometry (2011)
  • Travel Award for the 8th Uppsala Conference on Electron Capture and Transfer Dissociation (2011)

其他

本课题组欢迎特聘研究员系列、博士后、研究生等优秀科研人员加入。