联系方式

办公电话:

电子邮箱:lihlin6@mail.sysu.edu.cn

办公地址:广州市广州大学城外环东路132号中山大学药学院412A室

简单介绍

李惠琳,博士,中山大学教授,博导。201712月通过中山大学百人计划进入中山大学药学院工作。其研究工作以高分辨质谱技术为主要手段,开发国际领先的Top-Down MS技术,在整体蛋白、蛋白质机器水平上实现对蛋白的测序、蛋白翻译后修饰位点及药物结合位点的精准定位,进而对蛋白质机器结构和功能及蛋白-药物作用机制进行深入研究。其研究成果发表在Nature Chemistry, Analytical Chemistry, J. Am. Soc. Mass Spectrom.等国际主流化学、分析、及质谱类杂志。

研究方向

1.  蛋白-药物作用机制

2.  蛋白质机器结构及功能解析

3.  蛋白翻译后修饰调控机制

4.  小分子药物结构解析

教育经历

20169月至20179月:博士后,法国遗传与分子细胞生物学研究所结构生物学系,合作导师:Bruno Kieffer & Marc-Andre Delsuc教授;

20131月至20161月:博士后,美国加州大学洛杉矶分校David Geffen医学院生物化学系,合作导师:Joseph Loo教授;

200910月至201212月:博士,英国华威大学化学系,导师:Peter B. O’Connor 教授;

20059月至200812月:硕士,中国科学院长春应用化学研究所,导师:刘志强 研究员;

19989月至20027月:学士,山东大学药学院

工作经历

20027月至20099月:研习员,助理研究员,中国科学院长春应用化学研究所

论著专利

1.       H Li*, HH Nguyen, RRO Loo, I Campuzano, JA Loo*. An integrated native mass spectrometry and top-down proteomics method that connects sequence to structure and function of macromolecular complexes.. Nat. Chem.2018, 10(2):139-148

2.       H Li*, Y Sheng, W McGee, M Cammarata, D Holden, JA Loo*. Structural Characterization of Native Proteins and Protein Complexes by Electron Ionization Dissociation. Anal. Chem. 2017. 89, 2731-2738

3.       I Campuzano, H Li, G Svitel, D Bagal, L Jia, R Kurzeja, H Xu, JA Loo*. Bacteriorhodopsin and Nanodisc Analysis by Orthogonal Acceleration Time-of-flight, Ion Cyclotron Resonance and OrbiTrap Analyzers. Anal. Chem. 2016. 88, 12427-12436.

4.       P Wongkongkathep, H Li, X Zhang, RRO Loo, R Julian and JA Loo*. Enhancing Disulfide Bond Cleavage by UV Excitation and Electron Capture Dissociation for Top-Down Mass Spectrometry. Int. J. Mass Spectrom. 2015. 390, 137-145.

5.       X Zhang, H Li, JA Loo, RR Julian*, B Moore, P Wongkongkathep, RRO Loo. Radical directed dissociation of peptides and proteins by IRMPD and SORI-CID with FTICR mass spectrometry. Rapid Commun. Mass Spectrom. 2014. 28, 2729-2734.

6.       H Li, P Wongkongkathep, SL Van Orden, RRO Loo, JA Loo*. Revealing Ligand Binding Sites and Quantifying Subunit Variants of Non-Covalent Bound Protein Complexes in a Single Native Top-Down FTICR MS Experiment. J. Am. Soc. Mass Spectrom. 2014. 25, 2060-2068.

7.       H Li, JJ Wolff, SL Van Orden, JA Loo*. Native Top-Down ESI-MS of 158 kDa Protein Complex by High Resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Anal. Chem. 2014, 86, 317-320 (Paper On the Cover)

8.       H Li, JR Snelling, MP Barrow, JH Scrivens, PJ Sadler, PB O’Connor*. Mass Spectrometric Strategies to Improve the Identification of Pt(II)-modification Sites on Peptides and proteins. J. Am. Soc. Mass Spectrom. 2014. 25, 1217-1227.

9.       Y Zhao, NJ Farrer, H Li, JS Butler, RJ McQuitty, A Habtemariam, F Wang, PJ Sadler*. De novo generation of singlet oxygen and ammine ligands by photoactivation of a platinum anticancer complex. Angew. Chem. 2013. 52, 1-6.

10.    Y Qi, H Li, RH Wills, P Perez-Hurtado, X Yu, DPA Kilgour, MP Barrow, C LinPB O’Connor*. Absorption-mode Fourier transform mass spectrometry: The effects of apodization and phasing on modified protein spectra. J. Am. Soc. Mass Spectrom. 2013, 24, 828-834.

11.    J Wei, H Li, MP BarrowPB O’Connor*. Structural Characterization of Chlorophyll a by High Resolution Tandem Mass Spectrometry. J. Am. Soc. Mass Spectrom. 2013. 24, 753-760.

12.    Y Qi, Z Liu, H Li, PJ Sadler, PB O'Connor*. Mapping the Protein-Binding Sites for Novel Iridium(III) Anticancer Complexes Using Electron Capture Dissociation. Rapid Commun. Mass Spectrom. 201327, 2028-2032.

13.    Y Zhao, JA Woods, NJ Farrer, KS Robinson, J Pracharova, J Kasparkova, O Novakova, H Li, L Salassa, AM Pizarro, GJ Clarkson, L Song, V Brabec, PJ Sadler*. Diazido mixed-amine platinum(IV) anticancer complexes activatable by visible-light form novel DNA adducts. Chem. Eur. J. 2013, 19, 9578-9591.

14.    H Li, PB O’Connor*.Electron Capture Dissociation of Disulfide, Sulfur-Selenium, and Diselenide Bound Peptides. J. Am. Soc. Mass Spectrom. 2012, 23, 2001-2010.

15.    H Li#, SA Wells#, JE Jimenez-Roldan, RA Römer, Y Zhao, PJ Sadler, PB O’Connor*. Protein Flexibility is Key to Cisplatin Cross-linking in Calmodulin. Prot. Sci. 2012. 21, 1269-1279.

16.    Y Qi, MP Barrow, H Li, JE Meier, SL Van Orden, CJ Thompson, PB O'Connor*. Absorption-mode: The Next Generation of Fourier Transform Mass Spectra. Anal. Chem. 2012. 84, 2923–2929.

17.    H Zhou, H Li, Z Zheng, F Song, J Xing, Z Liu*, S Liu*. Screening for α-Glucosidase Inhibitors from Coptidis-rehmanniae Herb Couple by Using Ultrafiltration Liquid Chromatography/Mass Spectrometry. J. Liq. Chrom. Rel. Technol. 2012, 35, 1-14.

18.    H Li, TY Lin, SL Van Orden, Y Zhao, MP Barrow, AM Pizarro, Y Qi, PJ Sadler, PB O'Connor*. Use of Top-down and Bottom-up Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Mapping Calmodulin Sites Modified by Platinum Anticancer Drugs. Anal. Chem. 2011, 83, 9507-9515.

19.    H Li, Y Zhao, HIA Phillips, Y Qi, TY Lin, PJ Sadler, PB O’Connor*. Mass Spectrometry Evidence for Cisplatin as a Protein Cross-linking Reagent. Anal. Chem. 2011, 83, 5369-5376.

20.    Y Qi, MP Barrow, SL Van Orden, CJ Thompson, H Li, P Perez-Hurtado, PB O'Connor*. Variation of the Fourier Transform Mass Spectra Phase Function with Experimental Parameters. Anal. Chem. 2011, 83, 8477-8483.

21.    W Liu, S Liu, H Li, F Song, Z Liu, S Liu*. Binding of Alpha 1-acid Glycoprotein with Aconitum Alkaloids: An Investigation by IF-MALDI-FT-MS. Rapid Commun. Mass Spectrom. 2011, 25, 973-978.

22.    S Liu, J Yan, H Li, F Song, Z Liu, Z Liu, S Liu. Studies on Chemical Constituents of Compound Indigowoad Root Granule by Mass Spectrometry. Chem. J. Chinese U. 2010, 31, 1137-1142.

23.    H Li, F Song, J Xing, Z Liu*, S Liu*. Screening and Structures Characterization of α-Glucosidase Inhibitors from Hawthorn Leaves Flavonoids Extract by Ultrafiltration LC-DAD-MSn and SORI-CID FT ICR MS. J. Am. Soc. Mass Spectrom. 2009, 20, 1496-1503.

24.    X Meng, H Li, F Song, C Liu, Z Liu, S Liu. Studies on Triterpenoids and Flavones in Glycyrrhiza uralensis Fisch. by HPLC-ESI-MSn and FT-ICR-MSn. Chinese J. Chem. 2009, 27, 299-305.

25.    H Li, F Song, Z Zheng, Z Liu*, S Liu*. Characterization of Oligsaccharides and Phenolid Acids in the Chinese Herb Tanshen by ESI-FT ICR MS. J. Mass Spectrom. 2008, 43, 1545-1552.

26.    H Yue, Z Pi, H Li, F Song, Z Liu, S Liu. Studies on the Stability of Diester-diterpenoid Alkaloids from the Genus Aconitum L. by High Performance Liquid Chromatography Combined with Electrospray Ionisation Tandem Mass Spectrometry (HPLC/ESI/MSn). Phytochem. Anal. 2008, 19, 141-147.

27.    Y Liu, Z Liu, H Li, F Song, S Liu. Studies on Diterpenoids Constituents from Euphorbia Kansui by Electrospray Ionization Multi-Stage Tandem Mass Spectrometry. Chem. J. Chinese U. 2008, 29, 1727-1735.

28.    X Qiu, M Cui, H Li, Z Liu, S Liu. Prompt Disulfide Fragmentations of Disulfide-containing Proteins in a Matrix-Assisted Laser Desorption/ Ionization Source. Rapid Commun. Mass Spectrom. 2007, 21, 3520-3525.

29.    Y Zhao, F Song, H Yue, X Guo, H Li, Z Liu, S Liu. Deoxyaconitine of Metabolite of Aconitine by Human Intestinal Bacteria and Electrospray Ionization Tandem Mass Spectrometry. Chem. J. Chinese U. 2007, 28, 2051-2055.

30.    H Dong, Z Liu, F Song, Z Yu, H Li, S Liu. Structural Analysis of Monoterpene Glycosides Extracted from Paeonia lactiflora Pall. Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and High-performance Liquid Chromatography/ Electrospray Ionization Tandem Mass Spectrometry. Rapid Commun. Mass Spectrom. 2007, 21, 3193-3199.

31.    S Wang, H Li, Z Liu, S Liu, B Cui. Studies on the Contents of Catechin and Epicatechin in Catechu by HPLC, Lishizhen Medicine and Materia Medica Research. 2006, 17, 490-491.

32.    H Dong, Z Liu, F Song, Z Yu, H Li, S Liu. Studies on Paeoniflorin by Electrospray Ionization Tandem Mass Spectrometry. Chem. J. Chinese U. 2006, 27, 2066-2069.

获奖情况

AOMSC Young Scientist Travel Award (2017)

ASMS Postdoctoral Career Development Award (2014)

2009-2012 Warwick Postgraduate Research Scholarship (WPRS) (2009-2012)

University of Warwick Departmental Studentships (2009-2012)

Travel Award for the 25th Lake Louise Tandem Mass Spectrometry Workshop (2012)

IMSF Travel Grant for the 19th International Mass Spectrometry Conference (2012)

BMSS Travel Award for the 59th ASMS Conference on Mass Spectrometry (2011)

Travel Award for the 8th Uppsala Conference on Electron Capture and Transfer Dissociation (2011)

 

其他

本课题组欢迎特聘研究员系列、博士后、研究生等优秀科研人员加入。